United States securities and exchange commission logo June 3, 2024 Krishna Vaddi Chief Executive Officer Prelude Therapeutics Incorporated 175 Innovation Boulevard Wilmington, DE 19805 Re: Prelude Therapeutics Incorporated Registration Statement on Form S-3 Filed May 30, 2024 File No. 333-279829 Dear Krishna Vaddi: This is to advise you that we have not reviewed and will not review your registration statement. Please refer to Rules 460 and 461 regarding requests for acceleration. We remind you that the company and its management are responsible for the accuracy and adequacy of their disclosures, notwithstanding any review, comments, action or absence of action by the staff. Please contact Jason Drory at 202-551-8342 with any questions. Sincerely, Division of Corporation Finance Office of Life Sciences cc: Bryan S. Keighery